Post Doctoral

Job Description Title: Postdoctoral Fellow Salary: $73, 588 Department: Medicine/Nephrology Physical work location: One Gustave L. Levy Place, Annenberg 23, New York, NY 10029 Name PI or Supervisor: John Cijiang He, MD PhD, , Web link to Lab: Web link to Department: Administrative Contact: Irene Beaulieu, , Responsibilities Details of Research Project: Diabetic kidney disease (DKD) is the leading cause of chronic kidney disease (CKD) worldwide. Endothelial cell (EC) dysfunction is a key event leading to the progression of DKD. Through unbiased screening of EC transcriptome in animal models with early DKD, we identified cholesterol 25-hydroxylase (CH25H) as one of the highly regulated genes in diabetic kidneys. CH25H is the main enzyme that hydroxylates cholesterol to generate 25-hydroxycholesterol (25-HC). CH25H is known to be highly expressed in the kidney cells but its role in renal cell biology and pathology has never been reported. Over the last several years, we have generated a large amount of preliminary data to support the critical role of CH25H/25-HC in the pathogenesis of DKD. Based on these preliminary data, we hypothesized that CH25H/25-HC has a major protective role in DKD via maintaining normal Golgi function in ECs by inhibiting the negative regulatory effect of ASAP1 on ARF4 . To test this hypothesis, we propose the following aims:Aim 1: Determine how CH25H/25-HC are regulated in ECs and how CH25H/25-HC contributes to Golgi dysfunction and EC injury in diabetic conditions. Aim 2: Determine the cell-specific effects of CH25H on the development of DKD by using inducible EC-specific CH25H knockout or overexpression mice. Aim 3: Determine the role of ARF4 and Asap1 in the regulation of Golgi function and EC injury in DKD in mice. Qualifications Technical Duties: (include any protocols) ? Perform in vitro cell cultures of glomerular endothelial cells and test whether overexpression or knockdown of CH25h, ASAP1, and ARF4 affects Golgi function in endothelial cells. ? We have generated transgenic mouse models with overexpression or knockdown of Ch25h, ASAP1, and ARF4 in endothelial cells. We will test whether these mice develop more or less kidney injury. ? Techniques include cell culture, western blot, qPCR, RNA-sequencing, genotyping of mice, mouse breading, kidney histology analysis, immunostaining, determination of urine albumin and renal function. Educational and other Requirements for the position: Postdoc should have medical training (MD equivalent) or basic science training and is familiar with kidney disease pathogenesis (Nephrology training). Experience Required: Candidate shouldhave basic training in medical research and know how to perform basic bench works and animal studies. Goals/Outcomes of the Research Project: The proposed studies will help us to understand the mechanisms of Golgi regulation in DKD and identify potential new targets to develop drugs for treatment of DKD. SPOC-UAW Local 4100 at Icahn School of Medicine (Post Docs), 036 - Renal MSH - ISM, Icahn School of Medicine EOE Minorities/Women/Disabled/Veterans The Mount Sinai Health System is an equal opportunity employer. We comply with applicable Federal civil rights laws and does not discriminate, exclude, or treat people differently on the basis of race, color, national origin, age, religion, disability, sex, sexual orientation, gender identity, or gender expression. We are passionately committed to addressing racism and its effects on our faculty, staff, students, trainees, patients, visitors, and the communities we serve. Our goal is for Mount Sinai to become an anti-racist health care and learning institution that intentionally addresses structural racism.? About Us Strength Through Diversity The Mount Sinai Health System believes that diversity, equity, and inclusion are key drivers for excellence. We share a common devotion to delivering exceptional patient care. When you join us, you become a part of Mount Sinai's unrivaled record of achievement, education, and advancement as we revolutionize medicine together. We invite you to participate actively as a part of the Mount Sinai Health System team by: Using a lens of equity in all aspects of patient care delivery, education, and research to promote policies and practices to allow opportunities for all to thrive and reach their potential. Serving as a role model confronting racist, sexist, or other inappropriate actions by speaking up, challenging exclusionary organizational practices, and standing side-by-side in support of colleagues who experience discrimination. Inspiring and fostering an environment of anti-racist behaviors among and between departments and co-workers. At Mount Sinai, our leaders strive to learn, empower others, and embrace change to further advance equity and improve the well-being of staff, patients, and the organization. We expect our leaders to embrace anti-racism, create a collaborative and respectful environment, and constructively disrupt the status quo to improve the system and enhance care for our patients. We work hard to create an inclusive, welcoming and nurturing work environment where all feel they are valued, belong and are able to advance professionally. Explore more about this opportunity and how you can help us write a new chapter in our history! "About the Mount Sinai Health System: Mount Sinai Health System is one of the largest academic medical systems in the New York metro area, with more than 43,000 employees working across eight hospitals, more than 400 outpatient practices, more than 300 labs, a school of nursing, and a leading school of medicine and graduate education. 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We are consistently ranked by U.S. News & World Report's Best Hospitals, receiving high "Honor Roll" status, and are highly ranked: No. 1 in Geriatrics and top 20 in Cardiology/Heart Surgery, Diabetes/Endocrinology, Gastroenterology/GI Surgery, Neurology/Neurosurgery, Orthopedics, Pulmonology/Lung Surgery, Rehabilitation, and Urology. New York Eye and Ear Infirmary of Mount Sinai is ranked No. 12 in Ophthalmology. U.S. News & World Report's "Best Children's Hospitals" ranks Mount Sinai Kravis Children's Hospital among the country's best in several pediatric specialties. The Icahn School of Medicine at Mount Sinai is ranked No. 14 nationwide in National Institutes of Health funding and in the 99th percentile in research dollars per investigator according to the Association of American Medical Colleges. Newsweek's "The World's Best Smart Hospitals" ranks The Mount Sinai Hospital as No. 1 in New York and in the top five globally, and Mount Sinai Morningside in the top 20 globally. The Mount Sinai Health System is an equal opportunity employer. We comply with applicable Federal civil rights laws and does not discriminate, exclude, or treat people differently on the basis of race, color, national origin, age, religion, disability, sex, sexual orientation, gender identity, or gender expression. We are passionately committed to addressing racism and its effects on our faculty, staff, students, trainees, patients, visitors, and the communities we serve. Our goal is for Mount Sinai to become an anti-racist health care and learning institution that intentionally addresses structural racism." EOE Minorities/Women/Disabled/Veterans

Created: 2024-11-11