Genome and Vector Engineering Scientist

About us:We are harnessing the world's best protein factory to create a world of healthy abundance. Our vision is to expand accessibility and affordability of therapeutics, transforming human health globally. Through pushing the boundaries of genetic engineering, we're exploiting living bioreactors to produce highly valuable complex proteins using existing industrial-scale infrastructure. Our platform can rapidly and scalably produce almost any protein, and provide a new environment for novel therapeutics.Role Summary:We are looking for a talented scientist with knowledge and passion for ambitious synthetic biology projects and advanced genome engineering for biomanufacturing in living animals. We will be building tools and methodologies for in-vivo animal genome engineering, and will develop workflows to manipulate large DNA fragments and genomes. You will work with a talented group of engineers and biologists committed to developing disruptive, novel protein production technologies.Key Responsibilities:Platform development:Design, innovate and iterate on our transgenic animal platformDevelop and improve methods for DNA assembly, integration, editing, screeningDesign and build enabling molecular and cellular tools for DNA/RNA delivery and perform high-throughput screens and molecular evolution of these tools, including LNPs, VLPs, AAVs, lenti- and adenoviral vectors, electroporation and chemical transfection in cell culture, organ explants, live embryos and animalsQuantify genome editing events using various molecular assaysCell culture:Assist in derivation, culture, editing and cryopreservation of non-adherent germ cells, stem cells and somatic cellsPerform routine cell culture and maintenanceScientific housekeeping:Analyze data, generate figures, report results.Present findings at R&D meetingsMaintain a thorough and detailed electronic lab notebookMaintain a clean and organized laboratory environmentRequired Qualifications:PhD/MS in synthetic biology, bioengineering, molecular biology, genetics or related field is required, or 3+ years hands-on expertise in genomic manipulation and cell biologySolid understanding and hands-on experience with current precision editing techniques, covering the design, optimization and verification of edits made with CRISPR-Cas9/HDR, recombinases and integrases, and other large construct integration methodsExtensive experience with viral vector design, optimization and useExperience with development of gene therapy toolsExperience with avian or mammalian cell culture, DNA/RNA transfection, bacterial culture, recombinant DNA cloning techniques, primer design and PCR, qPCR, gel electrophoresis, nucleic acid extraction and purificationSelf-motivated, proactive, works well under pressure and deadlines, detail-orientedEnthusiasm for learning new skills and taking on new responsibilitiesStrong written and oral communication skills, as well as creative problem-solving skillsStrong emotional intelligence and passion for building relationships across all levels of an organization.Enjoys the fast-paced startup environment and teamworkPreferred Qualifications:High throughput screening experience or automation methodsExperience with protein purification and analysisExperience optimizing derivation, culture, gene editing and cryopreservation of stem cells, germ cells, PGCs, and/or non-adherent cell linesExperience with flow cytometry, immunostaining, and fluorescent microscopyExperience with dPCR and/or NGS library preparationWhy us?We are on a mission to reimagine the way in which complex biological molecules are produced to transform everything from cosmetics to therapeutics. Here, you will play a pivotal role in an early-stage, fast-growing company, working alongside leading scientists and entrepreneurs. You will have the opportunity to participate in the enormous potential upside while having a huge impact on the world. We're building a future of healthy abundance, one living bioreactor at a time. To do so, we're bringing together some of the best talent in the world - including you. We're very serious about what we do, but also serious about work-life balance, being decent people, and loving being at work.

Created: 2025-03-06